Spatial Transcriptomics Uncovers Hybrid, Pro-Inflammatory and Pro-Fibrotic Cellular Niches in Pulmonary Granuloma of Patients with Chronic Sarcoidosis - PubMed
5 hours ago
- #Pulmonary Fibrosis
- #Spatial Transcriptomics
- #Sarcoidosis
- Spatial transcriptomics reveals hybrid pro-inflammatory and pro-fibrotic cellular niches in pulmonary granulomas of chronic sarcoidosis patients.
- CD68+ macrophages form the central niche, surrounded by CD3+ T cells, CD20+ B cells, and COL3A1+ fibroblasts.
- Central macrophages express both pro-fibrotic (SPP1, CHIT1, CHI3L1) and pro-inflammatory genes, indicating a hybrid phenotype.
- Fibroblast niches show higher collagen and CTHRC1 expression, linked to pro-fibrotic lung remodeling.
- Ligand-receptor analysis identifies interactions supporting granuloma persistence through IFN-γ stimulation and collagen production.