Yinhuo Decoction attenuates depression-like behaviors and promotes M2 microglia polarization by regulating PI3K/Akt/NF-κB signaling pathway in a murine model of menopausal depression - PubMed
4 hours ago
- #Microglia polarization
- #Menopausal depression
- #Traditional Chinese Medicine
- Yinhuo Decoction (YHD) attenuates depression-like behaviors in a murine model of menopausal depression.
- YHD promotes M2 microglia polarization by regulating the PI3K/Akt/NF-κB signaling pathway.
- Seven YHD-derived components were identified in cerebrospinal fluid using UHPLC-LTQ-Orbitrap MS.
- Network pharmacology analysis suggested the PI3K/Akt/NF-κB pathway as a key mechanistic target.
- Core monomeric constituents (echinacoside, schisandrin B, verbascoside) modulate the pathway in LPS+ATP-stimulated BV2 microglia.
- Molecular docking and CETSA confirmed direct binding of these compounds to PI3K, with verbascoside showing the highest affinity.
- PI3K inhibitor LY294002 reversed verbascoside's effects on the PI3K/Akt/NF-κB pathway.