Investigating the relationship between ATP synthase and the TCA cycle by crosslinking mass spectrometry - PubMed
4 hours ago
- #ATP synthase
- #TCA cycle
- #mitochondria
- Study uses crosslinking mass spectrometry to explore ATP synthase interactions with TCA cycle enzymes in mitochondria.
- Finds ATP synthase's F₁ head forms extensive contacts with TCA cycle enzymes, linking oxidative phosphorylation and central carbon metabolism.
- Loss of LRPPRC protein destabilizes ATP synthase and increases F₁-TCA cycle interactions in mouse hearts.
- ATP synthase dysfunction promotes binding of inhibitory factor ATIF1, shifting it to an energy-preserving state.
- Suggests impaired mitochondrial gene expression leads to ATP synthase remodeling as an adaptation to bioenergetic stress.