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Diagnostic Accuracy of Liquid-Based Biomarkers for Detecting and Risk Stratifying Upper Tract Urothelial Cancers: A Diagnostic Test Systematic Review by the EAU Guidelines UTUC Panel - PubMed

3 days ago
  • #UTUC diagnosis
  • #Risk stratification
  • #Liquid biomarkers
  • Liquid-based biomarkers (LBBs) from urine and blood are emerging tools for diagnosing and risk stratifying upper tract urothelial carcinoma (UTUC).
  • A systematic review of 32 studies from 2010-2024 evaluated LBBs, excluding urine cytology, for diagnostic accuracy and prediction of high-grade or ≥pT2 disease.
  • Urine biomarkers like RNA panels, DNA methylation panels (e.g., GDF15, TMEFF2, VIM), Bladder Care Index, NRN1, ONECUT, and protein-based panels (BTA-STAT, BTA, survivin) showed favorable diagnostic profiles, though evidence certainty ranges from low to very low.
  • Upper-tract urine assay EpiCheck demonstrated modest accuracy (sensitivity 65-83%, specificity 79-83%), but strong performance for high-grade disease.
  • Blood biomarkers, particularly circulating tumor DNA (ctDNA) with plasma copy number burden >6.5, achieved high accuracy for predicting ≥pT2 and high-grade disease (sensitivity 71%, specificity 94%) with low certainty evidence.
  • RNA, DNA methylation, protein-based, and ctDNA assays show promise, but current evidence is limited, requiring large prospective cohorts and biomarker-driven trials before clinical integration.