Hijacking Actin-Pre-Liquid-Liquid Phase Separation Suppresses Malignant Tumor Invasion and Growth - PubMed
4 days ago
- #liquid-liquid phase separation
- #cytoskeleton
- #cancer therapy
- The cytoskeleton's role in cell migration and proliferation involves liquid-liquid phase separation (LLPS) of proteins.
- A storage and regulatory mechanism of cytoplasmic G-actin via LLPS has been discovered.
- A cross-linker, 4Arm-Pen, was developed to alter actin bioaggregate formation using orthogonal disulfide chemistry.
- 4Arm-Pen cross-links G-actin, impedes droplet formation, and affects F-actin polymerization kinetics, reducing cell invasiveness.
- Cross-linking G-actin depletes its availability, rendering cancer cells immotile and altering cellular mechanical parameters.
- This research offers new insights into modulating G-actin LLPS for potential cancer therapies.