Discovery of a [4Fe-4S] cluster in the PRRSV Nsp1α leader protease reveals host-virus interplay in its downstream functions - PubMed
5 hours ago
- #PRRSV
- #iron-sulfur cluster
- #antiviral strategy
- PRRSV Nsp1α leader protease contains a zinc-finger domain that primarily binds a [4Fe-4S] cluster, not just zinc.
- The Fe-S cluster, but not zinc, allosterically regulates the protease activity of Nsp1α.
- Nsp1α interacts with host iron-sulfur cluster assembly machinery via CIAO1 and competes with Fe-S carrier CIAO3.
- This discovery redefines Nsp1α as an Fe-S-dependent viral protein and suggests new antiviral strategies targeting metal cofactors.