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Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus netwo

9 hours ago
  • #Enteroviruses
  • #Next-Generation Sequencing
  • #Viral Genomics
  • Next-Generation Sequencing (NGS) offers comprehensive full-genome analysis for enterovirus detection and characterization, surpassing current molecular methods like PCR and partial VP1 sequencing.
  • Implementation of NGS faces challenges in standardizing wet-lab (laboratory workflows) and dry-lab (bioinformatics analyses) practices, which are not widely accessible in many laboratories.
  • The European non-polio enterovirus network (ENPEN) provides expert recommendations to harmonize NGS use in clinical, public health, and research settings for improved virus management and epidemiological surveillance.