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Small-molecule binding and sensing with a designed protein family - PubMed

5 hours ago
  • #protein design
  • #deep learning
  • #small-molecule sensing
  • Combined deep learning and physics-based methods to create a protein family with diverse, designable pockets for small-molecule binding.
  • Computationally designed binders for six small-molecule targets achieved nanomolar to low micromolar affinities with atomic-level accuracy.
  • Used a designed cortisol binder to develop a chemically induced dimerization (CID) system for a cortisol biosensor.
  • Demonstrates the potential of the NTF2 fold and deep learning in designing sensors for analytical, environmental, and biomedical applications.
  • Authors have filed provisional patent applications related to the designed protein sequences, indicating commercial interest.