ShineGAL4 drivers for tissue and cell-type specific optogenetics in Drosophila - PubMed
5 hours ago
- #Gene Expression
- #Drosophila
- #Optogenetics
- ShineGAL4 is an optogenetic split-GAL4 system enabling precise spatiotemporal gene manipulation in Drosophila.
- 14 widely used GAL4 drivers were converted into ShineGAL4 counterparts using CRISPR knock-in (HACK).
- ShineGAL4 allows light-induced gene expression in specific tissues and cell types like fat body, muscles, neurons, and glia.
- An optogenetic cassette was developed for photoactivation of GAL4 in 'silent' FLP-out clones.
- The toolset provides unprecedented control over gene expression in diverse Drosophila tissues and cell types.