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Decoding the spatiotemporal development of human meninges - PubMed

2 hours ago
  • #meningeal development
  • #spatial transcriptomics
  • #neuro-immunity
  • Used single-cell spatiotemporal transcriptomics from 6–23 gestational weeks to study human meninges development.
  • Identified asynchronous development of meningeal layers, with pia mater forming first.
  • Defined layer-specific fibroblast states expressing barrier, neurotransmitter, synapse, and lipid metabolism genes.
  • Characterized spatiotemporal heterogeneity in meningeal immune cells, including a meningeal-specific macrophage population.
  • Found pia mater recruits and organizes immune cells via CXCL12-CXCR4 signaling, particularly macrophages in leptomeninges.
  • Discovered Trem2+ macrophages regulate development of Cajal-Retzius cells in cerebral cortex.
  • Established a spatiotemporal framework for human meningeal development and uncovered neuro-immune interactions.
  • Identified potential therapeutic targets based on findings.