Nucleophagy is promoted by two autophagy receptors and inhibited by chromatin-nuclear envelope tethering in fission yeast - PubMed
6 hours ago
- #nucleophagy
- #nuclear envelope
- #autophagy receptors
- The study identifies Npr1 as a nucleophagy receptor in fission yeast, which works redundantly with Epr1 to promote nitrogen starvation-induced nucleophagy.
- Npr1 is an Atg8-binding multi-transmembrane protein located on the outer nuclear membrane, and both receptors co-localize with Atg8 at nuclear envelope protrusions during nucleophagy.
- In the absence of both Npr1 and Epr1, starved cells show abnormal nuclear morphology and reduced survival, indicating their critical role in nucleophagy.
- Nucleophagy involves the formation of outward protrusions from the nuclear envelope that detach to form autophagosomes containing nuclear cargo.
- Artificially enhancing chromatin association with the inner nuclear membrane prevents detachment of these protrusions, leading to abortive nucleophagy, which may protect chromatin from degradation.