A cell cycle-dependent transition of acetylation to phosphorylation regulates timely centrosome maturation - PubMed
5 hours ago
- #phosphorylation
- #centrosome maturation
- #cell cycle
- Centrosomes undergo maturation in G2/M phases to ensure proper spindle assembly and chromosome segregation.
- Polo-like kinase 1 (PLK1) is essential for centrosome maturation, but its timely recruitment mechanism was unclear.
- RNF40, a ubiquitin E3 ligase, is phosphorylated by CDK1 in G2/M phases, enabling PLK1 binding and centrosome maturation.
- RNF40 transitions from acetylation in interphase to phosphorylation in late G2/M, activating the CDK1-RNF40-PLK1 cascade.
- Disrupting RNF40 modifications (acetylation or phosphorylation) impairs PLK1 localization, microtubule nucleation, and spindle assembly, leading to mitotic catastrophe.
- The acetylation-to-phosphorylation transition of RNF40 ensures timely centrosome maturation and chromosome segregation.