A BRI1-CNGC12 phosphorylation module links hormone signaling to manganese homeostasis in plants - PubMed
4 hours ago
- #CNGC12
- #BRI1
- #Mn homeostasis
- Manganese (Mn) toxicity in acidic or waterlogged soils severely impacts crop productivity.
- High-Mn stress triggers Ca2+ signals that regulate Mn homeostasis, but the mechanism is unclear.
- Cyclic nucleotide-gated channels CNGC11/12 are essential for Mn tolerance; mutants show hypersensitivity to Mn.
- The brassinosteroid (BR) receptor BRI1 interacts with CNGC12 and phosphorylates Ser22, critical for channel activation.
- BRI1 mutants exhibit impaired Mn-induced Ca2+ signaling and heightened Mn sensitivity.
- Mn stress rapidly activates BRI1 kinase, peaking within minutes, and gates CNGC12-dependent Ca2+ currents.
- Exogenous brassinolide treatment enhances high-Mn-induced Ca2+ signaling, BRI1-mediated CNGC12 phosphorylation, and Mn tolerance.
- Mutations in BRI1 or CNGC12 impair CPK5-dependent phosphorylation of MTP8 and NRAMP1 endocytosis.
- The BRI1-CNGC12 module links BR signaling to Ca2+-dependent Mn detoxification, revealing hormone regulation of ion stress adaptation.