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Trihexyl phosphate exposure disrupts mitophagy and activates mtDNA-cGAS-STING signaling to drive pyroptosis and steroidogenic impairment in Leydig cells - PubMed

5 hours ago
  • #Mitochondrial dysfunction
  • #Pyroptosis
  • #Steroidogenesis
  • Trihexyl phosphate (THP) exposure reduces serum testosterone in male mice without altering LH/FSH levels.
  • THP disrupts PINK1/Parkin-mediated mitophagy, leading to mitochondrial dysfunction and mtDNA leakage.
  • The leaked mtDNA activates the cGAS-STING-TBK1-IRF3 pathway, triggering NLRP3 inflammasome signaling and pyroptosis.
  • Steroidogenic genes/proteins (NR5A1, SCARB1, STAR) are downregulated, and CYP11A1 + Leydig cell populations diminish.
  • Interventions like urolithin A and STING knockdown mitigate THP-induced testosterone suppression.
  • The study suggests mitophagy enhancement and cGAS-STING inhibition as potential therapeutic strategies.