Identification of mitochondria-related key genes in type 2 diabetes mellitus and elucidation of the Zhimu-Huangbai herb Pair's mechanism: an integrated approach of bioinformatics, machine learning, an
10 hours ago
- #herbal medicine mechanism
- #mitochondrial genes
- #type 2 diabetes
- Mitochondrial damage is key in type 2 diabetes mellitus (T2DM) pathogenesis, affecting energy metabolism and insulin sensitivity.
- The Zhimu-Huangbai herb pair (ZB) shows efficacy in T2DM, but its molecular mechanisms were unclear.
- Bioinformatics and machine learning identified eight mitochondria-related genes linked to ZB treatment in T2DM.
- Three key genes (BCAT2, CASP8, EPHX2) were screened via machine learning, forming diagnostic models with AUC >0.7.
- ZB treatment in INS-1 cells improved viability, reduced apoptosis and ROS, and enhanced mitochondrial function.
- ZB regulated mitochondrial-related genes (upregulating UCP2, BCAT2; downregulating CASP8, EPHX2) and activated the AMPK-SIRT1-PGC-1α pathway.
- The study provides insights for T2DM diagnosis and elucidates ZB's therapeutic mechanism involving mitochondrial regulation.