Editing strigolactone hormone receptor for robust antiviral silencing in rice - PubMed
6 hours ago
- #RNA silencing
- #strigolactones
- #plant antiviral immunity
- The siRNA pathway provides broad-spectrum antiviral defense through RNA silencing, which virulent infections must suppress.
- Strigolactone (SL) hormone signaling enhances antiviral silencing in rice by activating RDR1 and RDR6 transcription.
- Rice grassy stunt virus (RGSV) protein P3 inhibits SL signaling by binding to the receptor DWARF14, preventing its interaction with DWARF3.
- Structural analysis shows that aspartic acid at position 102 (D102) in DWARF14 is critical for P3 binding but not for SL perception.
- A D102N mutation in DWARF14, introduced via cytosine base editing (CBE), confers RGSV resistance by preventing viral suppression of SL signaling.
- This study presents a transgene-free method for engineering disease resistance by editing the SL receptor to evade pathogen defense suppression.