Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesis - PubMed
7 days ago
- #epithelial-mesenchymal interaction
- #regenerative dentistry
- #dentinogenesis
- Human dentin is secreted by odontoblasts and provides tooth support and sensory function.
- Dentin defects are common and irreparable beyond a critical threshold.
- Human dentin develops from dental papilla (DP) cells guided by dental epithelium (DE).
- A human tooth development atlas was created using single-cell RNA sequencing and spatial transcriptomics.
- The atlas reveals DE orchestrates DP differentiation via a WNT-NOTCH sequential activation model.
- DLX6-AS1+ DP cells respond to dental epithelial signals and can be isolated from adult DPSCs.
- DLX6-AS1+ DPSCs successfully generate tubular dentin in an in vivo disease model.
- This research provides insights into human tooth development and regenerative dental tissue repair.