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Lock-equipped six-helix DNA bundle-mediated siSTING delivery ameliorates Alzheimer's disease via cGAS-STING inhibition - PubMed

6 hours ago
  • #Alzheimer's disease
  • #siRNA delivery
  • #DNA nanotechnology
  • Alzheimer's disease (AD) progression is linked to microglial inflammation via the cGAS-STING pathway.
  • siSTING (small interfering RNA targeting STING) is a therapeutic to silence STING but faces challenges like serum degradation and poor BBB penetration.
  • A lock-equipped six-helix DNA bundle was developed for siSTING delivery, protecting it until it reaches AD lesions.
  • The DNA bundle releases siSTING in response to high CD64 expression at the lesion site.
  • This system suppresses cGAS-STING activation, reduces Aβ plaque deposition, and promotes microglial polarization from M1 to M2.
  • The treatment improved memory and learning deficits in AD model mice, offering a novel targeted therapy for AD.