High-throughput chemical proteomics workflow for profiling protein citrullination dynamics - PubMed
2 months ago
- #citrullination
- #post-translational modification
- #proteomics
- Development of a high-throughput chemical proteomics workflow for profiling protein citrullination dynamics.
- Improved specificity and throughput with glyoxal-based derivatization and a cleavable biotin linker for efficient peptide enrichment.
- > 10-fold increase in detection of citrullinated peptides at sub-0.1% abundance.
- Application to primary human neutrophils reveals dynamic regulation of citrullination by PAD4 inhibitor GSK484.
- Identification of a 'core citrullinome' conserved across stimuli, including response to Candida albicans.
- Extensive citrullination of linker histone H1 and structural proteins suggests remodeling of cell architecture during NET formation.