Bufei Jianspi formula alleviates intestinal epithelial barrier injury by inhibition of calpain 2 in COPD rat - PubMed
6 hours ago
- #Gut-Lung Axis
- #COPD
- #Traditional Chinese Medicine
- Bufei Jianspi Formula (BJF) alleviates intestinal epithelial barrier injury in COPD rats by inhibiting calpain 2 (CAPN2).
- BJF improves lung function, reduces pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), and mitigates oxidative stress in COPD rats.
- BJF restores protease-anti-protease balance by suppressing MMP-9 and MMP-12 while upregulating TIMP-1.
- BJF enhances tight junction protein expression, reducing intestinal permeability and inflammation.
- DARTS and CETSA confirm BJF binds directly to CAPN2; CAPN2 overexpression diminishes BJF's protective effects.
- Tangeretin and baohuoside I are identified as key BJF constituents with high CAPN2 binding affinity.
- BJF's protective effects on intestinal barrier integrity are validated in vitro using LPS-treated Caco-2 cells.