Discovery of human gut phage-encoded anti-CRISPR proteins unveils diverse mechanisms for phages to evade type II CRISPR immunity - PubMed
7 hours ago
- #phage biology
- #anti-CRISPR
- #gut microbiome
- Discovery of 651 phage-encoded anti-CRISPR (Acr) candidates targeting type II CRISPR systems in the human gut.
- Verification of 36 Acr candidates through plasmid interference and plaque assays, confirming CRISPR-Cas inhibitory activity.
- Mechanistic insights into five Acrs, including AcrIIB-1, revealing distinct inhibition strategies.
- Identification of 213 Acr candidates (GutAcraca) with structural convergence and dual functionality in transcription regulation and CRISPR-Cas inhibition.
- Widespread distribution of GutAcraca across microbial species, detected in 26% of species.
- Potential applications of these Acrs as biotechnology tools.