Identification and Validation of ALOX15 as a Robust Diagnostic Biomarker for Obesity-Associated Asthma: An Integrative Bioinformatic and Experimental Study - PubMed
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- #obesity-associated asthma
- #ALOX15 biomarker
- #bioinformatics validation
- The study identified ALOX15 as a key hub gene in obesity-associated asthma through integrative bioinformatics analysis.
- Machine learning algorithms (LASSO, Random Forest, SVM-RFE) and SHAP analysis confirmed ALOX15 as the most robust diagnostic candidate among nine identified genes.
- Validation in a high-fat diet-induced asthma mouse model showed increased ALOX15 expression correlating with exacerbated pulmonary inflammation and airway remodeling.
- Functional analyses linked ALOX15 to eosinophil infiltration, arachidonic acid metabolism, NF-κB signaling, and lipid metabolism pathways.
- ALOX15 may serve as a diagnostic biomarker and therapeutic target for obesity-associated asthma, bridging metabolic dysfunction to pro-inflammatory responses.