Single-Cell RNA-Sequencing Reveals Cachectic Satellite Cell Population in Muscle of Male Mice With Cancer Cachexia - PubMed
4 hours ago
- #cancer cachexia
- #single-cell RNA sequencing
- #satellite cells
- Single-cell RNA-sequencing in male mice with Lewis-lung carcinoma tumors identified a novel cachexia-associated satellite cell subcluster, comprising 71.1% of satellite cells at 3.5 weeks, linked to increased cell size and activation.
- Cancer cachexia led to significant body and lean mass decreases from 2.5 weeks, with muscle cross-sectional area reduced by 14.81% at 3.5 weeks compared to sham controls.
- Immune cell populations, including CD11b+, Ly6Clow, Ly6Chigh, F4/80+CD206-, and F4/80+CD206+, increased dramatically at 2 weeks before returning to baseline, while PDGFRα+ fibro-adipogenic progenitors rose and CD31+ endothelial cells declined.
- The cachexia-associated satellite cell subcluster showed enrichment in signaling pathways like IL-17, TNF, p53, NF-κB, FoxO, and JAK-STAT, which are implicated in satellite cell dysfunction during cachexia.
- Cell communication analyses indicated a general decline in intercellular signaling in cachexia, except for monocytes/macrophages, and reduced adhesion-related signaling in satellite cells, potentially explaining differentiation defects.