Single-cell network analysis reveals gene expression programs for Arabidopsis epidermis development and metabolism - PubMed
5 hours ago
- #Arabidopsis
- #epidermis development
- #single-cell RNA-seq
- Single-cell network analysis reveals gene expression programs for Arabidopsis epidermis development and metabolism.
- Integrated 11 public single-cell RNA-seq datasets of Arabidopsis shoots and leaves, extracting 30,247 epidermal cells.
- Constructed a single-cell gene co-expression network, AtEpidermisGGM, using the SingleCellGGM algorithm.
- Identified 120 co-expression modules (GEPs) delineating key developmental and metabolism processes of epidermal lineages.
- Spatial-temporal analysis revealed GEPs associated with L1-layer epidermis, stomatal development stages, and guard cell functioning.
- Discovered and validated roles of DMS1/2 in regulating stomatal density and DGC1/2/3 in controlling stomatal movement.
- AtEpidermisGGM provides a comprehensive map of epidermal gene programs, uncovering molecular modules for epidermal biology.