Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulator - PubMed
8 hours ago
- #CRISPR screening
- #UBE3C
- #MYC regulation
- A genome-wide CRISPR-Cas9 screen identified regulators of endogenous MYC expression in multiple myeloma.
- UBE3C, an E3 ubiquitin ligase, was found to repress MYC, while its paralogs UBE3A and UBE3B did not affect MYC levels.
- The Mediator complex subunit MED30 was identified as an activator of MYC expression.
- The screening system used an endogenous MYC-EGFP reporter cell line for quantitative assessment of MYC levels.