Responses of intestinal organoids to infection by Mycobacterium avium resemble symptoms observed in Crohn's disease - PubMed
7 days ago
- #Mycobacterium avium
- #Crohn's disease
- #intestinal organoids
- Crohn's disease (CD) is a chronic inflammatory bowel disease (IBD).
- Mycobacterium avium, causing Johne's disease in ruminants, is a potential trigger for CD due to shared pathology.
- A mouse small intestinal organoid (mSIO) model was established to study M. avium infection and CD-related inflammation.
- Infected mSIOs were analyzed using confocal microscopy, block-face scanning electron microscopy, and macrophage co-culture.
- RNA-seq and GSEA revealed strong induction of inflammatory genes and pro-inflammatory pathways.
- Comparative analysis with CD-humanized mouse data showed overlapping gene expression and IBD signaling pathway enrichment.
- Mmp7 was identified as a common marker in both models, linking infection to CD-like pathology.
- The study provides high-resolution insights into gut cell responses to mycobacterial infection.