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OTUB1 stabilizes PRRSV matrix protein through a non-canonical deubiquitination mechanism to promote viral replication - PubMed

5 hours ago
  • #OTUB1
  • #Deubiquitination
  • #PRRSV
  • PRRSV M protein is degraded via the ubiquitin-proteasome system, and the host deubiquitinase OTUB1 stabilizes it to promote viral replication.
  • OTUB1 binds to M's C-terminal ectodomain, removes K48-linked ubiquitin chains, and stabilizes M primarily by sequestering the E2 enzyme UBE2D2, not through canonical catalytic activity.
  • OTUB1 depletion reduces PRRSV replication in cells; its stabilizing effect is conserved across PRRSV strains, including emerging variants, suggesting OTUB1 as an antiviral target.