Identification of Ferroptosis-Related Hub Genes and Immune Infiltration Landscape in Chronic Kidney Disease via Bioinformatics and Experimental Verification - PubMed
6 hours ago
- #chronic kidney disease
- #bioinformatics
- #ferroptosis
- Chronic kidney disease (CKD) is a serious global health issue with increasing incidence.
- Ferroptosis plays a crucial role in kidney diseases, but its mechanism in CKD is not well understood.
- The study identified 7 ferroptosis-related hub genes (NNMT, GDF15, ACSL1, DLD, NFE2L2, PARP1, NR4A1) using bioinformatics tools like WGCNA, PPI, and GeneMANIA.
- These hub genes were validated and showed a clear link to renal function deterioration.
- ROC analysis demonstrated excellent diagnostic efficacy for these genes.
- Gene set enrichment analysis (GSEA) suggested these genes affect CKD through posttranscriptional modifications, immune dysfunction, and cell cycle dysregulation.
- CKD samples showed elevated levels of CD8+ T cells and M0 macrophages, while memory B cells, resting memory CD4+ T cells, Tregs, and resting mast cells decreased.
- In vitro experiments confirmed upregulation of NFE2L2, NNMT, and GDF15 in TGF-β1-treated HK-2 cells.
- The study suggests these hub genes as potential biomarkers and novel targets for CKD diagnosis and treatment.