CryoWriter: a robotic solution for improved Cryo-EM grid preparation - PubMed
4 hours ago
- #Cryo-EM
- #Microfluidic Technology
- #Robotic Grid Preparation
- CryoWriter is a robotic solution for cryo-EM grid preparation that uses blotting-free microfluidic technology.
- It writes nanoliter volumes onto EM grids via capillary-writing in spiral or line patterns, using minimal sample volumes.
- The system enables high-quality grids and near-atomic reconstructions for specimens like TMV, apoferritin, and TRPM4.
- Programmable deposition modes include double-writing to boost particle density and two-line writing for on-grid mixing for time-resolved studies.
- In challenging cases such as NrS-1 DNA polymerase, CryoWriter reduces orientation bias, leading to more isotropic reconstructions compared to conventional blotting.
- CryoWriter offers a versatile platform for reproducible low-volume grid preparation and supports on-grid biochemical workflows.