N4-acetylcytidine in LncRNA Gm26917 Promotes Translation in Female Germline Stem Cells by Recruiting Ribosomal Protein mRNA via EEF1A1 - PubMed
7 hours ago
- #Female Germline Stem Cells
- #N4-acetylcytidine
- #Translation Efficiency
- N4-acetylcytidine (ac4C) modification in lncRNA Gm26917 plays a crucial role in female germline stem cell (FGSC) maintenance.
- Depletion of ac4C modification impairs FGSC viability, proliferation, and promotes differentiation and apoptosis.
- ac4C modification in Gm26917 stabilizes its interaction with ribosomal protein mRNA Rpl10 via EEF1A1, enhancing protein synthesis.
- Reduction in RPL10 levels is due to impaired Gm26917-Rpl10 interaction rather than direct ac4C modification.
- Gm26917 or Rpl10 knockdown disrupts FGSC fate, while Rpl10 overexpression rescues translation efficiency defects.
- EEF1A1 disruption weakens the Gm26917-Rpl10 interaction and decreases mRNA translation efficiency.
- The study identifies a novel ac4C-Gm26917-EEF1A1-Rpl10 axis critical for FGSC maintenance and germ cell development.