Resolving synaptic events using subsynaptically targeted GCaMP8 variants - PubMed
5 hours ago
- #Drosophila neuroscience
- #synaptic calcium imaging
- #GCaMP8
- Engineered next-generation GCaMP8-based indicators for synaptic compartments in Drosophila neuromuscular junction.
- Developed Python-based analysis program, CaFire, for automated quantification of Ca²+ signals.
- Presynaptic GCaMP8m sensor shows superior sensitivity and similar kinetics to chemical dyes.
- Active zone-targeted GCaMP8m sensor reports differences in Ca²+ between release sites.
- Postsynaptic GCaMP8m detects quantal events with resolution comparable to electrophysiology.
- GCaMP8 sensors achieve speed and sensitivity previously only possible with chemical dyes or electrophysiology.