Bisphenol TMC Impaired Mouse Oocyte Maturation by Disrupting Spindle Assembly via NEK3-Mediated Microtubule Acetylation - PubMed
4 hours ago
- #Oocyte Maturation
- #Spindle Assembly
- #BPTMC
- Bisphenol TMC (BPTMC) is a novel bisphenol A substitute with estrogenic activity, detected in the environment and human tissues.
- BPTMC exposure reduced mouse oocyte maturation in a dose-dependent manner, lowering polar body extrusion (MII oocytes) rates.
- It disrupted cytoplasmic maturation, causing abnormal mitochondria, increased reactive oxygen species (ROS), and apoptosis.
- Defects in cytoplasmic maturation led to reduced fertilization and impaired embryonic development (lower pronuclei and 2-cell embryo formation).
- Mechanistically, BPTMC impaired spindle assembly by reducing microtubule acetylation via downregulation of NEK3 protein.