Monocytes acquire a tumor-associated IL1B program upon encountering patient-derived colon cancer organoids - PubMed
11 hours ago
- #Tumor Microenvironment
- #Colon Cancer
- #Monocytes
- Monocytes acquire a tumor-associated IL1B program when exposed to patient-derived colon cancer organoids (PDOs).
- Tumor-associated macrophages (TAMs) and monocytes in colorectal cancer (CRC) influence the tumor microenvironment and immune responses.
- Co-cultures of human monocytes and PDOs mimic myeloid/tumor cell interactions, revealing distinct phenotypic changes in monocytes.
- Single-cell RNA sequencing shows PDO-exposed monocytes resemble IL1B-programmed monocytes found in CRC tumor tissues.
- PDO-derived mediators induce chemokine production (CXCL2, CXCL5, CXCL7) and impair MHC class II-mediated antigen presentation in monocytes.
- PDO-exposed monocytes show reduced inflammatory response upon TLR stimulation.
- The study provides a platform to study cancer cell-monocyte interactions and myeloid function in cancer.