Single-oocyte full-length isoform sequencing unveils the impact of transposable elements on RNA diversity and stability - PubMed
6 hours ago
- #isoform diversity
- #oocyte transcriptomics
- #transposable elements
- Single-cell full-length isoform sequencing identifies numerous novel transcripts in human and mouse oocytes, revealing isoform diversity during maturation.
- Novel isoforms, especially novel-not-in-catalog (NNC) types, have specific functions; e.g., ARHGAP18 isoforms colocalize with microtubules, and their knockdown disrupts oocyte maturation.
- Approximately 30% of NNC isoforms are derived from transposable elements, which enhance isoform stability, highlighting co-option for RNA diversity and stability.