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Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets - PubMed

5 hours ago
  • #microfluidics
  • #biomolecular condensates
  • #stress granules
  • Stress granules (SGs) are biomolecular condensates that form in mammalian cells during stress, with dysregulation linked to age-related diseases.
  • An emulsion-based microfluidics method using 'cytosolic extracts-in-oil droplets' (CEODs) mimics the cytosolic environment for SG formation under physiological conditions.
  • CEODs enabled the discovery of seven new SG components involved in signaling pathways, offering a cost-effective screening platform for future therapeutic studies.