BMAL1 modulates glutamine supply to control hematopoietic stem and progenitor cell expansion - PubMed
5 hours ago
- #Glutamine Metabolism
- #Circadian Clock
- #Hematopoiesis
- BMAL1, a core circadian clock component, is expressed in zebrafish CHT endothelial cells (ECs) and non-cell autonomously affects hematopoietic stem and progenitor cells (HSPCs).
- Endothelial-specific dominant-negative Bmal1a zebrafish lines show increased HSPC numbers due to enhanced proliferation.
- RNA-sequencing reveals downregulation of glud1a in dominant-negative bmal1a ECs, leading to elevated glutamine levels in the CHT.
- The bmal1a-glud1a-glutamine pathway is identified as a regulator of HSPC expansion.
- This glutamine synthesis pathway is conserved in the mouse fetal liver niche, with hepatocytes as the likely glutamine source.
- EC BMAL1 controls bioavailable glutamine for HSPCs by regulating glutamine synthesis genes.