Development and application of G4-Flame as a visual biosensor for G4-DNA - PubMed
5 hours ago
- #Cancer Biomarker
- #G4-DNA
- #Biosensor
- G4-Flame is a genetically encoded fluorescent biosensor designed for visualizing G4-DNA dynamics in living systems.
- G4-DNA is a noncanonical tetrahelical nucleic acid structure critical in genomic regulation and disease pathogenesis.
- G4-Flame utilizes circularly permuted YFP (cpYFP) for real-time, high-resolution detection of G4-DNA with specificity across diverse conformations.
- Nuclear G4-DNA levels peak during the S phase of the cell cycle, while mitochondrial G4-DNA suppresses mitochondrial-encoded gene expression.
- Serum analysis showed significantly elevated G4-DNA levels in cancer patients compared to healthy controls.
- G4-Flame has potential as a biomarker for early cancer detection and advances research in G4-DNA spatiotemporal regulation.