Design of binder proteins specific to IL-13 - PubMed
6 hours ago
- #Interleukin-13
- #Minibinder
- #Protein Design
- Researchers used structure-based computational design to create compact minibinder proteins targeting IL-13, based on the IL-13-tralokinumab crystal structure.
- Five designed constructs were expressed in E. coli, with binder1 showing the strongest nanomolar binding affinity and most potent antagonism in IL-13-dependent proliferation assays (IC50 = 56.39 nM).
- Binder1 effectively suppressed STAT6 phosphorylation and reduced IL-13-induced pro-inflammatory gene expression in lung epithelial cells, indicating potential for topical immunotherapy in IL-13-related disorders.