Scalable single-cell total RNA sequencing unifies coding and noncoding transcriptomics - PubMed
6 hours ago
- #single-cell RNA sequencing
- #transcriptomics
- #noncoding RNA
- Developed a scalable single-cell total RNA sequencing framework (TotalX) that captures both coding and noncoding RNA, unifying transcriptomics.
- Applied to human brain development, revealing cell-type and temporal-specific expression programs across neuronal and non-neuronal lineages.
- Identified enrichment and target anticorrelation of microRNA MIR137 in Cajal-Retzius neurons, linked to schizophrenia and intellectual disability.
- Used TotalX on human blood cells to uncover transcriptional modules combining coding and noncoding RNAs and tRNA dynamics.
- Captured non-adenylated viral transcripts in dengue-infected hepatocytes, distinguishing infection states for improved atlas-scale understanding.