Developmentally Inspired, Mechanical-Metabolic Dual Gradient Osteochondral Constructs Bridging Regeneration and Therapeutic Screening - PubMed
12 hours ago
- #osteochondral interface
- #3D bioprinting
- #drug screening
- Developmentally inspired dual-gradient 3D-printed osteochondral constructs mimic native stiffness and metabolic microenvironments.
- Human bone marrow-derived mesenchymal stem cell spheroids are used to promote chondrogenesis in soft, hypoxic niches and osteogenesis in stiff, vascular-rich regions.
- The integration of mechanical and metabolic gradients enhances extracellular matrix formation beyond single-cue designs.
- The anisotropic architecture maintains cartilage-bone crosstalk and enables drug response assessment for osteoarthritis modeling and therapy screening.
- This model bridges functional regeneration with preclinical drug screening, offering a physiologically relevant platform for osteochondral repair and disease research.