Whole-organ and whole-body 3D atlases enable cellome-wide profiling - PubMed
6 hours ago
- #cellomics
- #3D imaging
- #tissue clearing
- Recent advancements in tissue clearing and light-sheet fluorescence microscopy enable whole-organ/body-scale analysis at single-cell resolution.
- The CUBIC Organ/Body Atlas provides 3D single-cell-resolution references for eleven adult mouse organs and a neonatal whole-mouse body.
- Optimized tissue clearing protocols and the exMOVIE imaging system were developed to achieve organ-/body-wide 3D imaging and cell nuclei detection.
- The atlas supports comparative analysis among multiple samples at single-cell resolution for organ development, disease state analysis, and whole-body immune cell profiling.
- The CUBIC Organ/Body Atlas advances systems-level understanding of organisms in physiological, developmental, and pathological processes.