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Miniaturized IL-2/anti-IL-2 immunocytokines selectively activate and support the in vivo persistence of regulatory T cells - PubMed

8 hours ago
  • #IL-2 Therapy
  • #Immunocytokine
  • #Regulatory T Cells
  • Miniaturized version of F5111 immunocytokine (miniF5111 IC) was created by fusing IL-2 to a single-chain variable fragment (scFv) of the F5111 antibody.
  • Binding and signaling studies showed miniF5111 IC selectively targets regulatory T cells (Tregs) over effector lymphocytes, similar to the full-length version.
  • In vivo, miniF5111 IC expanded Tregs but had a reduced serum half-life compared to the full-length F5111 IC.
  • Engineered Tregs expressing miniF5111 IC demonstrated prolonged persistence and sustained FOXP3 expression after adoptive transfer.
  • The miniaturized immunocytokine is a promising platform for targeted immune cell activation, with therapeutic potential in autoimmune diseases and transplant rejection.