Organization of mouse prefrontal cortex subnetwork revealed by spatial single-cell multi-omic analysis of SPIDER-Seq - PubMed
6 hours ago
- #spatial-omics
- #SPIDER-Seq
- #prefrontal cortex
- SPIDER-Seq technique combines viral barcoding tracing with single-cell sequencing and spatial-omics.
- The study delineates an integrated single-cell spatial molecular, cellular, anatomic, and projectomic atlas of the mouse prefrontal cortex (PFC).
- Projectomic and transcriptomic cell clusters in the PFC display distinct modular organization principles but are coordinately configured.
- Projection neurons in the PFC gradiently occupy territories aligning with their wiring patterns and show higher co-projection probability to downstream nuclei with reciprocal circuit connections.
- The study integrates projectomic atlas with neurotransmitter/neuropeptide profiles to demonstrate PFC neural signal transmission networks.
- Machine learning was used to predict neuron projections by combining gene profiles and spatial information, including projections of fear recall engram neurons.