3D pentaculture model unveils malignant cell-driven macrophage polarization in high-grade serous ovarian cancer - PubMed
5 hours ago
- #ovarian cancer
- #tumor microenvironment
- #3D cell culture
- A 3D pentaculture model was developed to replicate the human tumor microenvironment (TME) of high-grade serous ovarian cancer (HGSOC).
- The model incorporates five human cell types: malignant HGSOC cells, primary fibroblasts, mesothelial cells, adipocytes, and monocytes that differentiate into macrophages without added cytokines.
- Macrophage clusters in the model mirror those found in patient HGSOC metastases, with proportions varying based on the malignant cell line used.
- The model enables analysis of how malignant cell heterogeneity (genomic, transcriptomic, proteomic) influences the TME and interactions with macrophages.
- Targeting 'do-not-eat-me' signals with anti-CD47 and anti-CD24 antibodies showed differential effects on macrophage activity and cancer cell viability, dependent on the malignant cell line.
- Real-time microscopy confirmed dynamic modulation of macrophage behavior, highlighting the model's utility for studying malignant cell control over TME elements, particularly myeloid interactions.