Selective, genetically induced increase in synaptic vesicle priming - PubMed
6 hours ago
- #Munc13-1 mutation
- #presynaptic plasticity
- #synaptic vesicle priming
- Developed a genetic approach to selectively increase synaptic vesicle (SV) priming by introducing a histidine-to-lysine mutation at position 567 of Munc13-1, enhancing its function.
- Combined mutation with Cre-dependent removal of wild-type allele to enable cell type-selective enhancement of Munc13-1, increasing excitatory postsynaptic current amplitude at hippocampal synapses solely through elevated release probability (Pv).
- Showed that increased Pv results from a higher proportion of well-primed SVs without altering fusion probability (Pfusion), release site number, or quantal size, as predicted by a sequential two-step priming model.
- Provided evidence that variability in presynaptic glutamate release is mainly due to differences in SV priming, highlighting priming as a key mechanism in functional synaptic diversity.