APOBEC3B regulates HPV replication by inducing R-loop formation and DNA damage - PubMed
5 hours ago
- #R-loop
- #APOBEC3B
- #HPV replication
- APOBEC3B (A3B) is a nuclear cytidine deaminase that positively regulates HPV replication and expression.
- A3B binds to cellular and viral chromatin at high R-loop regions, including HPV URR and early polyadenylation sites.
- A3B depletion reduces R-loop levels by ~50%, impairing viral transcription and increasing immune gene expression (e.g., OASL, IL6, IRF1).
- A3B regulates R-loops at transcriptional start (TSS) and termination sites (TTS) of cellular genes and HPV URR.
- A3B depletion leads to a >50% reduction in DNA breaks and alters DNA damage repair protein expression.
- A3B induces R-loop formation and DNA damage in HPV-positive cells, influencing viral and cellular gene expression.