Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning - PubMed
14 hours ago
- #genome-editing
- #TnpB
- #CRISPR
- Engineered TnpB genome editors identified for plants and human cells.
- TnpB is a family of RNA-guided endonucleases linked to prokaryotic transposons.
- Most TnpBs lack robust gene-editing activity, but activating mutations were discovered.
- Two enhanced TnpB variants were identified, increasing editing efficiency in human cells and plants.
- Engineered variants achieved up to 55% insertion and deletion frequencies in Nicotiana benthamiana.
- The study highlights critical elements for regulating TnpB endonuclease activity.
- Patents related to the findings have been submitted by some authors.
- Funding was provided by the National Institutes of Health and the National Science Foundation.