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A Calixsalan-based ionizable lipid for macrophage-targeted Trem2 siRNA delivery attenuates atherosclerosis - PubMed

5 hours ago
  • #Atherosclerosis
  • #Macrophage
  • #siRNA
  • A novel Calixsalan-based ionizable lipid enables macrophage-targeted lipid nanoparticles (LNPs) for siRNA delivery.
  • Targeting Trem2 via siRNA attenuates atherosclerosis by silencing macrophage Trem2 and suppressing pro-atherogenic pathways.
  • Circulating soluble Trem2 (sTrem2) is identified as an independent clinical predictor of coronary artery disease.
  • Macrophages promote vascular smooth muscle cell proliferation and migration via PDGF-BB secretion in a KLF4-dependent manner.
  • Intravenous administration of targeted LNPs in mice significantly reduced plaque burden, validating Trem2 silencing as a therapeutic strategy.