Reactive oxygen species in lipid nanoparticle-based nucleic acid delivery: Mechanisms and engineering - PubMed
5 hours ago
- #Reactive oxygen species
- #Lipid nanoparticle
- #mRNA delivery
- Reactive oxygen species (ROS) have dual roles in lipid nanoparticle (LNP)-mediated nucleic acid delivery.
- ROS influence various stages of delivery, including lipid stability, nucleic acid integrity, endosomal escape, and immune activation.
- The review discusses ROS distribution across the LNP delivery lifecycle and their roles in processes like endosomal membrane damage and inflammasome activation.
- ROS-based engineering strategies are categorized into ROS-responsive, ROS-regulatory, and combinatorial approaches.
- ROS-responsive strategies use redox stimuli for controlled cargo release.
- ROS-regulatory strategies incorporate antioxidants to modulate redox homeostasis and preserve nucleic acid stability.
- Combinatorial strategies integrate additional modalities like photodynamic or chemotherapeutic agents to balance efficiency and biosafety.
- Understanding ROS-mediated regulation aids in designing next-generation LNPs with improved efficiency and reduced toxicity.