Live-cell imaging of RNA dynamics using bright and stable fluorescent RNAs - PubMed
5 hours ago
- #fluorescent RNAs
- #RNA imaging
- #live-cell imaging
- Live-cell imaging of RNA dynamics is crucial for understanding RNA functions.
- Fluorescent RNAs (FRs) like Pepper, Clivia, and Okra enable high-resolution RNA imaging.
- FRs offer advantages such as brightness, photostability, and low ion dependence.
- Protocol includes tagging RNA with aptamers for visualization in bacteria and mammalian cells.
- Multiplexed RNA imaging and super-resolution live imaging are detailed in the protocol.
- The process takes 5-7 days from cloning to results analysis.